pLeo695 vector (V005023)

LOCUS       40924_28142        8537 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLeo695, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming 
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
  PUBMED    29686358
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 8537)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8537)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..1184
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    1200..1204
                     /label=SfiI
                     /note="SfiI"
     sig_peptide     1243..1305
                     /label=Ig-kappa leader
                     /note="leader sequence from mouse immunoglobulin kappa 
                     light
                     chain"
     misc_feature    1312..1689
                     /label=anti RR120
                     /note="anti RR120"
     misc_feature    1697..2445
                     /label=EpoR-D1_D2_TM
                     /note="EpoR-D1_D2_TM"
     misc_feature    1975..1977
                     /label=F93A
                     /note="F93A"
     misc_feature    2455..3283
                     /label=IL6ST
                     /note="IL6ST"
     misc_feature    2806..2808
                     /label=Y759A
                     /note="Y759A"
     polyA_signal    3380..3604
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(3653..3672)
                     /label=itr
                     /note="itr"
     promoter        4036..4140
                     /label=AmpR promoter
     CDS             4141..4998
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5172..5760
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6187..6206
                     /label=itr
                     /note="itr"
     polyA_signal    complement(6323..6378)
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(6546..7142)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    complement(7143..7202)
                     /label=P2A
                     /note="P2A"
     CDS             complement(7143..7199)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7209..7907)
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     regulatory      complement(7921..8532)
                     /label=RPBSA
                     /note="RPBSA"
                     /regulatory_class="promoter"
     regulatory      7921..8532
                     /label=RPBSA
                     /note="RPBSA"
                     /regulatory_class="promoter"