Basic Vector Information
- Vector Name:
- pLeo410
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6580 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
pLeo410 vector Map
pLeo410 vector Sequence
LOCUS 40924_28007 6580 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLeo410, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6580)
AUTHORS Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
TITLE Generalized extracellular molecule sensor platform for programming
cellular behavior
JOURNAL Nat. Chem. Biol. 14 (7), 723-729 (2018)
PUBMED 29686358
REFERENCE 2 (bases 1 to 6580)
AUTHORS Scheller L, Strittmatter T.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
4058, Switzerland
REFERENCE 3 (bases 1 to 6580)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6580)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6580
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 21..39
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 40..64
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 79..101
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
misc_feature 84..100
/label=T7_transl_en_RBS
/note="T7_transl_en_RBS"
CDS 108..815
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 846..902
/label=GCN4 tag
/note="GCN4 tag"
CDS 846..902
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 918..974
/label=GCN4 tag
/note="GCN4 tag"
CDS 918..974
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 963..965
/label=R
/note="R"
misc_feature 990..1046
/label=GCN4 tag
/note="GCN4 tag"
CDS 990..1046
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 1062..1118
/label=GCN4 tag
/note="GCN4 tag"
CDS 1062..1118
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 1134..1190
/label=GCN4 tag
/note="GCN4 tag"
CDS 1134..1190
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 1206..1262
/label=GCN4 tag
/note="GCN4 tag"
CDS 1206..1262
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 1278..1334
/label=GCN4 tag
/note="GCN4 tag"
CDS 1278..1334
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
misc_feature 1350..1406
/label=GCN4 tag
/note="GCN4 tag"
CDS 1350..1406
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1425..1445
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 1461..1478
/label=6xHis
/note="6xHis affinity tag"
terminator 1545..1592
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1629..2084
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(2180..2992)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 3114..3702
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(3888..4030)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4135..4323)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(5098..5119)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(5135..6214)
/label=lacI
/note="lac repressor"
promoter complement(6215..6292)
/label=lacI promoter
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