Basic Vector Information
- Vector Name:
- pLEMY
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5693 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Lv H, Gong Z.
- Promoter:
- LEU2
pLEMY vector Vector Map
pLEMY vector Sequence
LOCUS 40924_27667 5693 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLEMY, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5693)
AUTHORS Lv H, Gong Z.
TITLE Cost-effective and repeatable gene targeting in fission yeast using
a cloning-based and modular method
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5693)
AUTHORS Lv H, Gong Z.
TITLE Direct Submission
JOURNAL Submitted (24-MAR-2012) School of Life Science, Fudan University,
Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE 3 (bases 1 to 5693)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5693)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(24-MAR-2012) School of Life Science, Fudan University, Room 618,
Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5693
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 235..253
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 352..381
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 385..414
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 418..447
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 451..480
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 484..513
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 517..546
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 550..579
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 583..612
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 616..645
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 649..678
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 682..711
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 715..744
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
regulatory 787..1279
/label=Saccharomyces cerevisiae Leu2 terminator
/note="Saccharomyces cerevisiae Leu2 terminator"
/regulatory_class="terminator"
promoter 1526..1931
/label=LEU2 promoter
CDS 1932..3023
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
regulatory 3027..3503
/label=Saccharomyces cerevisiae Leu2 terminator
/note="Saccharomyces cerevisiae Leu2 terminator"
/regulatory_class="terminator"
promoter complement(3582..3600)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(3858..4446)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4620..5477)
/label=AmpR
/note="beta-lactamase"
promoter complement(5478..5582)
/label=AmpR promoter
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