Basic Vector Information
- Vector Name:
- pLDUNB
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12242 bp
- Type:
- Load driver vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R.
- Promoter:
- TEF1
pLDUNB vector Vector Map
pLDUNB vector Sequence
LOCUS V005067 12242 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V005067
VERSION V005067
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 12242)
AUTHORS Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R.
TITLE A load driver device for engineering modularity in biological
networks
JOURNAL Nat. Biotechnol. 32 (12), 1268-1275 (2014)
PUBMED 25419739
REFERENCE 2 (bases 1 to 12242)
AUTHORS Mishra D, Rivera-Ortiz PM, Lin A, Del Vecchio D, Weiss R.
TITLE Direct Submission
JOURNAL Submitted (02-SEP-2014) Synthetic Biology Center, MIT, 500
Technology Square, NE47, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 12242)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12242)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
Biotechnol."; date: "2014"; volume: "32"; issue: "12"; pages:
"1268-1275"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-SEP-2014) Synthetic Biology Center, MIT, 500 Technology Square,
NE47, Cambridge, MA 02139, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12242
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 50..542
/label="HO-R"
/note="HO-R"
promoter 569..599
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 653..1309
/label="CmR"
/note="chloramphenicol acetyltransferase"
rep_origin 1487..2075
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2383..2866
/label="HO-L"
/note="HO-L"
protein_bind 2966..2986
/label="attB4"
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 3040..3439
/label="TEF1 promoter"
/note="promoter for EF-1-alpha"
primer_bind 3443..3459
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3485..3509
/label="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3512..4516
/label="rtTA"
/note="tetracycline-controlled transactivator, comprising a
fusion of the reverse tetracycline repressor rTetR with the
C-terminal activation domain of herpes simplex virus VP16"
protein_bind complement(4521..4545)
/gene="mutant version of attB"
/label="attB2"
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(4523..4544)
/label="attB2"
/note="attB2"
terminator 4568..4755
/label="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature 4858..4879
/label="attB4"
/note="attB4"
protein_bind 4859..4879
/gene="mutant version of attB"
/label="attB4"
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 4933..5203
/label="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
regulatory 5229..5385
/regulatory_class="promoter"
protein_bind 5395..5419
/gene="mutant version of attB"
/label="attB1"
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 5396..5416
/label="attP1"
/note="attP1"
CDS 5428..7293
/gene="SKN7"
/label="Transcription factor SKN7"
/note="Transcription factor SKN7 from Saccharomyces
cerevisiae (strain ATCC 204508 / S288c). Accession#:
P38889"
protein_bind complement(7301..7325)
/gene="mutant version of attB"
/label="attB2"
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(7303..7324)
/label="attB2"
/note="attB2"
terminator 7340..7587
/label="CYC1 terminator"
/note="transcription terminator for CYC1"
misc_feature 7688..7709
/label="attB4"
/note="attB4"
protein_bind 7689..7709
/gene="mutant version of attB"
/label="attB4"
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
regulatory 7743..8475
/regulatory_class="promoter"
misc_feature 8159..8198
/label="SKN7 binding site"
/note="SKN7 binding site"
misc_feature 8199..8238
/label="SKN7 binding site"
/note="SKN7 binding site"
protein_bind 8487..8511
/gene="mutant version of attB"
/label="attB1"
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 8487..8508
/label="attP1"
/note="attP1"
CDS 8519..9232
/label="GFP"
/note="green fluorescent protein"
protein_bind complement(9240..9264)
/gene="mutant version of attB"
/label="attB2"
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(9242..9263)
/label="attB2"
/note="attB2"
terminator 9274..9471
/label="TEF terminator"
/note="Ashbya gossypii TEF terminator"
misc_feature 9640..9661
/label="attB4"
/note="attB4"
protein_bind 9641..9661
/gene="mutant version of attB"
/label="attB4"
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
regulatory 9711..10118
/regulatory_class="promoter"
promoter 9716..10114
/gene="S. cerevisiae TEF1"
/label="TEF1 promoter"
/note="promoter for EF-1-alpha"
primer_bind 10118..10134
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 10160..10184
/label="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 10193..10999
/label="KanR"
/note="aminoglycoside phosphotransferase"
protein_bind complement(11004..11028)
/label="attB2"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 11033..11157
/label="PHO5 Terminator"
/note="PHO5 Terminator"
/regulatory_class="terminator"
CDS 11314..12120
/label="KanR"
/note="aminoglycoside phosphotransferase"
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