Basic Vector Information
- Vector Name:
- pL0D_2-R
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5694 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, Mueller-Roeber B.
- Promoter:
- LYS2
pL0D_2-R vector Map
pL0D_2-R vector Sequence
LOCUS 40924_27354 5694 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pL0D_2-R, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5694) AUTHORS Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, Mueller-Roeber B. TITLE AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies JOURNAL Nucleic Acids Res. (2017) In press PUBMED 28130422 REFERENCE 2 (bases 1 to 5694) AUTHORS Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, Mueller-Roeber B. TITLE Direct Submission JOURNAL Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, Brandenburg 14476, Germany REFERENCE 3 (bases 1 to 5694) TITLE Direct Submission REFERENCE 4 (bases 1 to 5694) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res. (2017) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, Brandenburg 14476, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5694 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(575..1375) /codon_start=1 /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(1376..1596) /label=URA3 promoter rep_origin complement(2195..3075) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" CDS complement(3424..4215) /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(4216..4320) /label=AmpR promoter misc_feature 4389..4428 /label=8 cutter set: AscI, SbfI, SwaI, FseI, PmeI /note="8 cutter set: AscI, SbfI, SwaI, FseI, PmeI" misc_feature 4429..4464 /label=homology region D2* /note="homology region D2*" CDS 4800..5102 /codon_start=1 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" promoter 5194..5433 /label=LYS2 promoter misc_feature 5434..5484 /label=homology region DR* /note="homology region DR*" misc_feature 5488..5527 /label=8 cutter set: SwaI, AscI, PmeI, SbfI, FseI /note="8 cutter set: SwaI, AscI, PmeI, SbfI, FseI" rep_origin 5553..5694 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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