pKZ2-attS-C2 vector (V005160)

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LOCUS       V005160                 9913 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005160
VERSION     V005160
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9913)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-FEB-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(956..1813)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1814..1918)
                     /label="AmpR promoter"
     primer_bind     2392..2408
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3456..4055
                     /codon_start=1
                     /gene="repC"
                     /product="temperature sensitive replication protein RepC"
                     /label="repC"
                     /protein_id="ASN64769.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            3456..4055
                     /gene="repC"
                     /label="repC"
     CDS             complement(4391..5014)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    5036..5054
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5121..5139
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     misc_feature    complement(5144..5716)
                     /label="antisense-secY"
                     /note="antisense-secY"
     CDS             6410..7057
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      7272..7370
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             7368..9128
                     /codon_start=1
                     /gene="ccrC2"
                     /product="cassette chromosome recombinase CcrC2"
                     /label="ccrC2"
                     /note="N-terminal attSCC tag"
                     /protein_id="ASN64772.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     gene            7368..9128
                     /gene="ccrC2"
                     /label="ccrC2"
     repeat_region   7382..7420
                     /gene="ccrC2"
                     /label="attSCC"
                     /note="attSCC"
     repeat_region   7399..7417
                     /gene="ccrC2"
                     /label="Direct repeat"
                     /note="Direct repeat"
     CDS             9415..9813
                     /codon_start=1
                     /gene="bleoR"
                     /product="bleomycin resistance protein"
                     /label="bleoR"
                     /protein_id="ASN64773.1"
                     /translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
                     HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
                     KDQWWDERDFAVIDPDNNLISFFQQIKS"
     gene            9415..9813
                     /gene="bleoR"
                     /label="bleoR"