pKU1021gps vector (V005166)

LOCUS       40924_27109        7308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKU1021gps DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Yamada Y, Kuzuyama T, Komatsu M, Shin-Ya K, Omura S, Cane DE, Ikeda 
            H.
  TITLE     Terpene synthases are widely distributed in bacteria
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (3), 857-862 (2015)
  PUBMED    25535391
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Ikeda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, 
            Kitasato Institute for Life Sciences; 1-15-1 Kitasato, Sagamihara, 
            Kanagawa 252-0373, Japan URL :http://avermitilis.ls.kitasato-u.ac.jp
REFERENCE   3  (bases 1 to 7308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "3"; pages: 
            "857-862"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2014) Contact:Haruo Ikeda Kitasato University, Kitasato 
            Institute for Life Sciences"; volume: " 1-15-1 Kitasato, Sagamihara,
            Kanagawa 252-0373, Japan URL :http"; pages: 
            "//avermitilis.ls.kitasato-u.ac.j"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             344..1135
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(1476..1585)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1639..1650)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    1721..1820
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             1830..3644
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     rep_origin      3867..4455
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4743..4764
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4779..4809
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4817..4833
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4841..4857
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4900..5967)
                     /codon_start=1
                     /gene="gps"
                     /product="geranyl diphosphate synthase"
                     /label=gps
                     /note="from Streptomyces sp."
                     /protein_id="BAP82233.1"
                     /translation="MTPDVTNDASQETVDLVRRTSADLLRRVEARIQSLLSAGHDTWAA
                     VDERAVVPIDALAELMASGGKRIRPAFCIAGHLAAGGDPDDSGVVAAGAALEMLHASLL
                     VHDDILDDSSQRRGLPTVHAKHTALHQDLGWQGESRRFGEGVGLLVGGLALTYADELMC
                     EAPATAIAEWNKLRSEVLIGQYMDVVAAAEFSVDPRLSRLIAVIKSGRYTIHRPLVIGA
                     NVAGRTDLDAAFAEYGEAVGEAFQLRDDLLDAFGDSAATGKPAGLDFTQHKMTLLLGWA
                     MQRDESIRALITEPGHSADDVRRHLLDTKVPDDVEQHIAGLVERGCKAITDAPIAPVWR
                     EELMEMANRVAYRDA"
     gene            complement(4900..5967)
                     /gene="gps"
                     /label=gps
     regulatory      complement(5971..6517)
                     /gene="rpsJp"
                     /note="promoter of gene encoding ribosomal protein S10;
                     from Streptomyces avermitilis MA-4680"
                     /regulatory_class="promoter"
     gene            complement(5971..6517)
                     /gene="rpsJp"
                     /label=rpsJp
     promoter        6522..6550
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6739..7269
                     /label=GmR
                     /note="gentamycin acetyltransferase"