pKT313 vector (V005169)

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LOCUS       40924_27094        6180 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal transformation vector pKT313, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     New plasmids allowing positive-negative selection for fungal 
            transformation and efficient Cre-loxP mediated marker recycling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Twaruschek K, Spoerhase P, Michlmayr H, Wiesenberger G, Adam G.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2018) Department of Applied Genetics and Cell 
            Biology, University of Natural Resources and Life Sciences Vienna 
            (BOKU), Konrad Lorenz Strasse 24, Tulln 3430, Austria
REFERENCE   3  (bases 1 to 6180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2018) Department of Applied Genetics and Cell Biology, 
            University of Natural Resources and Life Sciences Vienna (BOKU), 
            Konrad Lorenz Strasse 24, Tulln 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      62..808
                     /note="pki; Trichoderma reesei pyruvate kinase I promoter; 
                     accession CP016233"
                     /regulatory_class="promoter"
     regulatory      215..224
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..1941
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     CDS             1978..3000
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      3022..3751
                     /note="cbh2; Trichoderma reesei cellobiohydrolase II
                     terminator; accession CP016238"
                     /regulatory_class="terminator"
     promoter        complement(3818..3836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4094..4682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4856..5713)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5714..5818)
                     /label=AmpR promoter
     promoter        6164..6180
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"