pEX-1 vector (V012525)

Download GeneBank File(.gb)

LOCUS       pEX-1.        4505 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) bacterial vector for inducible expression of an
            untagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    pEX-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the BseRI and MluI sites. The 
            BseRI site is compatible with SgfI sites from other 
            PrecisionShuttle(TM) vectors.
FEATURES             Location/Qualifiers
     source          1..4505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        218..236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    237..261
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             265..287
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    288..382
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      421..467
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        713..790
                     /label=lacI promoter
     CDS             791..1870
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1886..1907
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1922..1952
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     1984..2000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2016..2032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2324..2912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3086..3943)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3944..4048)
                     /label=AmpR promoter
     rep_origin      4075..4505
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"