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pEX-K vector (V012521)

Basic Vector Information

Vector Name:
pEX-K
Antibiotic Resistance:
Kanamycin
Length:
2507 bp
Type:
Cloning Vectors
Replication origin:
ori
Source/Author:
Eurofins Genomics
Selection Marker:
Neomycin/G418(Geneticin)
Copy Number:
High copy number

pEX-K vector Map

Copy Sequence View Map
pEX-K2507 bp600120018002400lac operatorlac promoterCAP binding siteoriNeoR/KanR

pEX-K vector Sequence

LOCUS       40924_18696        2507 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Streamlined bacterial cloning vector with a kanamycin resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Eurofins Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(41..57)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(65..95)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(110..131)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(419..1007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1309..2100
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

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