pGEM-7Zf(-) vector (V012477)

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LOCUS       pGEM-7Zf(-).        2998 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector for in vitro RNA synthesis, ssDNA production, and 
            generation of unidirectional deletions. Identical to pGEM(R)?7Zf(+) 
            except for the orientation of the f1 origin.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-7Zf(-)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..110
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(122..140)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(158..174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(182..198)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(206..236)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(251..272)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(560..1148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1322..2179)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2180..2284)
                     /label=AmpR promoter
     rep_origin      2363..2818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2959..2975
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2982..2998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"