pGEM-9Zf(-) vector (V012476)

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LOCUS       pGEM-9Zf(-).        2912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a compact MCS for in vitro RNA synthesis, ssDNA 
            production, and generation of unidirectional deletions.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-9Zf(-)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..53
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(54..72)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(102..118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(126..142)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(150..180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(195..216)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(504..1092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1266..2123)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2124..2228)
                     /label=AmpR promoter
     rep_origin      2255..2710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2851..2867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2896..2912
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"