pGEM-T vector (V012472)

LOCUS       pGEM-T.        3000 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Parental vector for TA cloning of PCR products. The insertion site 
            is flanked by BstZI sites. This vector is also known as 
            pGEM(R)-5Zf(+).
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize at EcoRV to create a TA cloning vector.
FEATURES             Location/Qualifiers
     source          1..3000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    10..113
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(125..143)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(161..177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(185..201)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(209..239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(254..275)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(563..1151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1325..2182)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2183..2287)
                     /label=AmpR promoter
     rep_origin      complement(2365..2820)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2961..2977
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2984..3000
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"