pGS-21a vector (V012470)

LOCUS       pGS-21a.        6169 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of 6xHis- and GST-tagged proteins. 
            Two 6xHis sequences are encoded in the vector.
ACCESSION   .
VERSION     .
KEYWORDS    pGS-21a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   GenScript
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..91
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             101..118
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             128..781
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             818..832
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    834..892
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             893..910
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      977..1024
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1061..1516
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1543..1647
                     /label=AmpR promoter
     CDS             1648..2505
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2679..3267
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3453..3595)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3700..3888)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(4663..4684)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4700..5779)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(5780..5857)
                     /label=lacI promoter