pMAL-c5G vector (V012451)

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LOCUS       pMAL-c5G.        5680 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible cytoplasmic expression of 
            maltose-binding protein (MBP) fusions with a Genenase(TM) I cleavage
            site.
ACCESSION   .
VERSION     .
KEYWORDS    pMAL-c5G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Riggs P.
  TITLE     Expression and purification of maltose-binding protein fusions.
  JOURNAL   Curr Protoc Mol Biol 2001;Chapter 16:Unit16.6.
  PUBMED    18265133
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol"; date: "2001"; volume: "Chapter 16"; pages: "Unit16.6"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1160
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1176..1197
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1406..1434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1528..2628
                     /label=MBP
                     /note="maltose binding protein from E. coli"
     CDS             2677..2694
                     /label=Genenase(TM) I site
                     /note="recognition and cleavage site for Genenase(TM) I, a 
                     subtilisin BPN' variant engineered for increased substrate 
                     specificity (Carter and Wells, 1987)"
     misc_feature    2692..2765
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      2766..2852
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2944..2971
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2998..3089
                     /label=AmpR promoter
     CDS             3090..3947
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4038..4626
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4999..5187)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"