pNEB193 vector (V012442)

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LOCUS       pNEB193.        2713 bp DNA     circular SYN 01-JAN-1980
DEFINITION  pUC19 derivative with additional sites for the 8-cutters AscI, PmeI,
            and PacI.
ACCESSION   .
VERSION     .
KEYWORDS    pNEB193
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..479
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(492..508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(516..532)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(540..570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(585..606)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(894..1482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1656..2513)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2514..2618)
                     /label=AmpR promoter