pRHA-113 vector (V012420)

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LOCUS       pRHA-113.        5837 bp DNA     circular SYN 01-JAN-1980
DEFINITION  pBR322-derived low copy number plasmid encoding the rhaB promoter 
            plus rhaR and rhaS genes, for rhamnose-inducible protein expression 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    pRHA-113
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, 
            Surber MW.
  TITLE     Toxic protein expression in Escherichia coli using a rhamnose-based 
            tightly regulated and tunable promoter system.
  JOURNAL   BioTechniques 2006;40:355-64.
  PUBMED    16568824
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Xbrane Bioscience
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2006"; volume: "40"; pages: "355-64"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     This plasmid sequence was reconstructed from the procedure described
            in the original paper. The plasmid size is slightly different than 
            reported.
FEATURES             Location/Qualifiers
     source          1..5837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(40..885)
                     /label=rhaR
                     /note="transcriptional activator of rhaR and rhaS"
     CDS             complement(962..1795)
                     /label=rhaS
                     /note="positive regulator of the rhaB promoter"
     promoter        1941..2059
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     misc_feature    2077..2127
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             3391..3579
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3684..3824
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4010..4598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4772..5629)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5630..5734)
                     /label=AmpR promoter