pSEAP2-Basic vector (V012413)

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LOCUS       pSEAP2-Basic.        4677 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless mammalian vector encoding secreted alkaline 
            phosphatase, for cloning promoter and enhancer elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSEAP2-Basic
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..72
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             74..1582
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(1640..1761)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2180..2768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2942..3799)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3800..3904)
                     /label=AmpR promoter
     rep_origin      3931..4386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4517..4565
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4579..4670
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"