pSF-Core vector (V012412)

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LOCUS       pSF-Core.        3734 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless backbone SnapFast(TM) cloning vector, on which all of 
            the other plasmids from Oxford Genetics are based.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-Core
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    273..358
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             309..314
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     319..325
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             323..325
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    377..387
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    425..435
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(467..588)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      702..749
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      779..915
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    960..1031
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             2578..2583
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             2591..3448
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3464..3600
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"