pSP64 Poly(A) vector (V012398)

LOCUS       pSP64_Poly(A).        3030 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector for in vitro transcription from the SP6 promoter with
            the addition of a synthetic 30-base poly(A) tail.
ACCESSION   .
VERSION     .
KEYWORDS    pSP64 Poly(A)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Use any MCS sites other than ApoI/EcoRI to generate a transcript 
            with a poly(A) tail.
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..91
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    54..83
                     /label=poly(A)
                     /note="poly(A)"
     primer_bind     complement(100..116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(124..140)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(148..178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(193..214)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(502..1090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1264..2121)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2122..2226)
                     /label=AmpR promoter
     promoter        3014..3030
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"