pSP72 vector (V012397)

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LOCUS       40924_41027        2462 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector for in vitro transcription using the SP6 and T7 RNA 
            polymerase promoters. Essentially identical to pSP73 except for the 
            orientation of the MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Krieg PA, Melton DA.
  TITLE     In vitro RNA synthesis with SP6 RNA polymerase.
  JOURNAL   Meth. Enzymol. 1987;155:397-415.
  PUBMED    2828872
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Meth. 
            Enzymol."; date: "1987"; volume: "155"; pages: "397-415"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(16..72)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(100..118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(376..964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1138..1995)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1996..2100)
                     /label=AmpR promoter
     promoter        2446..2462
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"