pSTBlue-1 vector (V012389)

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LOCUS       pSTBlue-1.        3851 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Multi-purpose bacterial cloning and expression vector with a 
            versatile MCS, T7 and SP6 promoters, and ampicillin and kanamycin 
            resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    pSTBlue-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    51..178
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(184..202)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(216..232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(373..828)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        855..959
                     /label=AmpR promoter
     CDS             960..1817
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             1966..2778
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2866..3454
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3742..3763
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3778..3808
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3816..3832
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."