pTrcHis2 B vector (V012379)

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LOCUS       pTrcHis2_B.        4404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of C-terminally 
            6xHis-tagged proteins. For other reading frames, use pTrcHis2 A or 
            pTrcHis2 C.
ACCESSION   .
VERSION     .
KEYWORDS    pTrcHis2 B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     misc_feature    346..354
                     /label=gene 10 translational enhancer
                     /note="gene 10 translational enhancer"
                     /note="translational enhancer from gene 10 of bacteriophage
                     T7 (Olins and Rangwala, 1989)"
     RBS             369..373
                     /note="ribosome binding site"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     misc_feature    411..470
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             469..498
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             514..531
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      757..843
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      935..962
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        982..1073
                     /label=AmpR promoter
     CDS             1074..1931
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2105..2693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2879..3019)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3205..3282
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3283..4362
                     /label=lacI
                     /note="lac repressor"