Basic Vector Information
- Vector Name:
- pAC155-pCR8-sgExpression
- Antibiotic Resistance:
- Streptomycin
- Length:
- 3178 bp
- Type:
- CRISPR Plasmids
- Replication origin:
- ori
- Source/Author:
- Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan
- Copy Number:
- High copy number
- Promoter:
- U6
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pAC155-pCR8-sgExpression vector Map
pAC155-pCR8-sgExpression vector Sequence
LOCUS pAC155-pCR8-sgEx 3178 bp DNA circular SYN 01-JAN-1980 DEFINITION Gateway(R) entry vector for expression of a single guide RNA (sgRNA) from the U6 promoter. ACCESSION . VERSION . KEYWORDS pAC155-pCR8-sgExpression SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3178) AUTHORS Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. TITLE Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. JOURNAL Cell Res. 2013;23:1163-71. PUBMED 23979020 REFERENCE 2 (bases 1 to 3178) AUTHORS Jaenisch Lab / Addgene #49045 TITLE Direct Submission REFERENCE 3 (bases 1 to 3178) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res."; date: "2013"; volume: "23"; pages: "1163-71" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3178 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(268..295) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 477..493 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 509..608 /label=attL1 /note="recombination site for the Gateway(R) LR reaction" terminator 698..703 /note="polIII terminator" /note="RNA polymerase III transcription terminator" misc_RNA complement(704..779) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter complement(806..1046) /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" protein_bind complement(1057..1156) /label=attL2 /note="recombination site for the Gateway(R) LR reaction" promoter complement(1174..1192) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1197..1213) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 1644..2432 /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" rep_origin 2528..3116 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
This page is informational only.