pAC155-pCR8-sgExpression vector (V012303)

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LOCUS       pAC155-pCR8-sgEx        3178 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) entry vector for expression of a single guide RNA (sgRNA)
            from the U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAC155-pCR8-sgExpression
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R.
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013;23:1163-71.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Jaenisch Lab / Addgene #49045
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3178)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res.";
            date: "2013"; volume: "23"; pages: "1163-71"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     477..493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    509..608
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      698..703
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     misc_RNA        complement(704..779)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(806..1046)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     protein_bind    complement(1057..1156)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1174..1192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1197..1213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1644..2432
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"