pAcGFP1-C2 vector (V012077)

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LOCUS       pAcGFP1-C2.        4735 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing AcGFP1 to the N-terminus of a partner protein. For
            other reading frames, use pAcGFP1-C1 or pAcGFP1-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pAcGFP1-C2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
     misc_feature    1334..1399
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1408..1418
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1523..1644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1651..2106)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2133..2237
                     /label=AmpR promoter
     promoter        2239..2596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2631..3422
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3657..3704
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4033..4621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"