Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012024 | pEGFP-C1 | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pEGFP-C1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4731 bp
- Type:
- Fluorescent Protein Genes & Plasmids
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- CMV
- Cloning Method:
- Enzyme Cut
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
- Expression Method:
- Transient
pEGFP-C1 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
References
- Payán-Bravo L, Fontalva S, Peñate X, Cases I, Guerrero-Martínez JA, Pareja-Sánchez Y, Odriozola-Gil Y, Lara E, Jimeno-González S, Suñé C, Muñoz-Centeno MC, Reyes JC, Chávez S. Human prefoldin modulates co-transcriptional pre-mRNA splicing. Nucleic Acids Res. 2021 Jun 21;49(11):6267-6280. doi: 10.1093/nar/gkab446
- Payán-Bravo L, Fontalva S, Peñate X, Cases I, Guerrero-Martínez JA, Pareja-Sánchez Y, Odriozola-Gil Y, Lara E, Jimeno-González S, Suñé C, Muñoz-Centeno MC, Reyes JC, Chávez S. Human prefoldin modulates co-transcriptional pre-mRNA splicing. Nucleic Acids Res. 2021 Jun 21;49(11):6267-6280. doi: 10.1093/nar/gkab446
- Medlej A, Mohammad Soltani B, Javad Mowla S, Hosseini S, Baharvand H. A novel miRNA located in the GATA4 gene regulates the expression of IGF-1R and AKT1/2 genes and controls cell proliferation. J Cell Biochem. 2020;121(5-6):3438-3450. doi:10.1002/jcb.29617
- Asselin L, Rivera Alvarez J, Heide S, et al. Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activity. Nat Commun. 2020;11(1):2441. doi:10.1038/s41467-020-16294-6
- Kim HJ, Kim HJ, Kim MK, et al. SPSB1 enhances ovarian cancer cell survival by destabilizing p21. Biochem Biophys Res Commun. 2019;510(3):364-369 https://doi.org/10.1016/j.bbrc.2019.01.088
- Namyanja, Monica & Xu, Zhi-Shen & Mugasa, Claire & Lun, Zhao-Rong & Matovu, Enock & Chen, Zhengjun & Lubega, George. (2019). Preliminary evaluation of a Trypanosoma brucei FG-GAP repeat containing protein of mitochondrial localization. AAS Open Research. 2. 165. 10.12688/aasopenres.12986.1. https://aasopenresearch.org/articles/2-165
- Abildgaard AB, Stein A, Nielsen SV, et al. Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome. Elife. 2019 https://doi.org/10.7554/eLife.49138
pEGFP-C1 vector Sequence
LOCUS Exported 4731 bp DNA circular SYN 26-AUG-2024
DEFINITION Vector for fusing EGFP to the N-terminus of a partner protein. For
other reading frames, use pEGFP-C2 or pEGFP-C3.
ACCESSION .
VERSION .
KEYWORDS pEGFP-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4731)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4731)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 4731)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4731
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label=EGFP
/note="enhanced GFP"
misc_feature 1330..1395
/label=MCS
/note="multiple cloning site"
misc_feature 1404..1414
/label=stop codons
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 1519..1640
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1647..2102)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2129..2233
/label=AmpR promoter
promoter 2235..2592
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2627..3418
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3653..3700
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4029..4617
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"