pEGFP-C3 vector (V012022)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012022	pEGFP-C3	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:: pEGFP-C3

Antibiotic Resistance:: Kanamycin

Length:: 4727 bp

Type:: Fluorescent Protein Genes & Plasmids

Replication origin:: ori

Source/Author:: Clontech

Selection Marker:: Neomycin/G418(Geneticin)

Copy Number:: High copy number

Promoter:: CMV

pEGFP-C3 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-C3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pEGFP-C3.        4727 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing EGFP to the N-terminus of a partner protein. For 
            other reading frames, use pEGFP-C1 or pEGFP-C2.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP-C3.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1335..1391
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
     misc_feature    1400..1410
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1515..1636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1643..2098)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2125..2229
                     /label=AmpR promoter
     promoter        2231..2588
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2623..3414
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3649..3696
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4025..4613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"