pKillerRed-C vector (V011986)

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LOCUS       pKillerRed-C.        4731 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing KillerRed to the N-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pKillerRed-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
     misc_feature    1330..1395
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
     polyA_signal    1519..1640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1647..2102)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2129..2233
                     /label=AmpR promoter
     promoter        2235..2592
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2627..3418
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3653..3700
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4029..4617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"