pLSSmOrange-C1 vector (V011981)

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LOCUS       pLSSmOrange-C1.        4716 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing LSSmOrange to the N-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pLSSmOrange-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV.
  TITLE     An orange fluorescent protein with a large Stokes shift for 
            single-excitation multicolor FCCS and FRET imaging.
  JOURNAL   J. Am. Chem. Soc. 2012;134:7913-23.
  PUBMED    22486524
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Verkhusha Lab
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Am. 
            Chem. Soc."; date: "2012"; volume: "134"; pages: "7913-23"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             607..1314
                     /label=LSSmOrange
                     /note="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
     misc_feature    1315..1380
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1389..1399
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1504..1625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1632..2087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2114..2218
                     /label=AmpR promoter
     promoter        2220..2577
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2612..3403
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3638..3685
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4014..4602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"