pRSET-CFP vector (V011940)

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LOCUS       pRSET-CFP.        3600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing CFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pRSET-CFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             59..81
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..91
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             101..118
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             122..154
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /label=epitope tag from a T7 major capsid protein
                     /note="T7 tag"
                     /translation="MASMTGGQQMG"
     CDS             158..181
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             209..925
                     /label=CFP
                     /note="cyan variant of GFP"
     terminator      1004..1051
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1148..1603
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1629..1733
                     /label=AmpR promoter
     CDS             1734..2591
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2765..3353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"