pAd/CMV/V5-DEST vector (V011866)

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LOCUS       V011866                36686 bp    DNA     circular UNA 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011866
VERSION     V011866
KEYWORDS    pAd CMV V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 36686)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 36686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..36686
                     /mol_type="genomic DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..341
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        731..1110
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1111..1314
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1359..1377
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1407..1531
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1963..2265)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(2610..3266)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3267..3369)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3547..3671)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3697..3738
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     polyA_signal    3822..3870
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             4152..4571
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(4637..5971)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(5743..9324)
                     /gene="POL"
                     /label="DNA polymerase"
                     /note="DNA polymerase from Human adenovirus C serotype 2.
                     Accession#: P03261"
     CDS             complement(9129..11132)
                     /gene="PTP"
                     /label="Preterminal protein"
                     /note="Preterminal protein from Human adenovirus C serotype
                     5. Accession#: P04499"
     CDS             11593..12837
                     /gene="L1"
                     /label="Packaging protein 3"
                     /note="Packaging protein 3 from Human adenovirus C serotype
                     5. Accession#: P04496"
     CDS             12861..14615
                     /note="Pre-hexon-linking protein IIIa from Human adenovirus
                     C serotype 5. Accession#: P12537"
                     /label="Pre-hexon-linking protein IIIa"
     CDS             14699..16411
                     /gene="L2"
                     /label="Penton protein"
                     /note="Penton protein from Human adenovirus C serotype 5.
                     Accession#: P12538"
     CDS             16421..17014
                     /gene="L2"
                     /label="Pre-histone-like nucleoprotein"
                     /note="Pre-histone-like nucleoprotein from Human adenovirus
                     C serotype 2. Accession#: P68950"
     CDS             17087..18190
                     /gene="L2"
                     /label="Core-capsid bridging protein"
                     /note="Core-capsid bridging protein from Human adenovirus C
                     serotype 5. Accession#: P24938"
     CDS             18221..18460
                     /note="Pre-core protein X from Human adenovirus C serotype
                     5. Accession#: Q2KS10"
                     /label="Pre-core protein X"
     CDS             18546..19295
                     /gene="L3"
                     /label="Pre-protein VI"
                     /note="Pre-protein VI from Human adenovirus C serotype 5.
                     Accession#: P24937"
     CDS             19385..22240
                     /gene="L3"
                     /label="Hexon protein"
                     /note="Hexon protein from Human adenovirus C serotype 5.
                     Accession#: P04133"
     CDS             22276..22887
                     /gene="L3"
                     /label="Protease"
                     /note="Protease from Human adenovirus C serotype 5.
                     Accession#: P03253"
     CDS             complement(22989..24575)
                     /gene="DBP"
                     /label="DNA-binding protein"
                     /note="DNA-binding protein from Human adenovirus C serotype
                     5. Accession#: P03265"
     CDS             24604..27024
                     /gene="L4"
                     /label="Shutoff protein"
                     /note="Shutoff protein from Human adenovirus C serotype 5.
                     Accession#: P24933"
     CDS             27717..28397
                     /gene="L4"
                     /label="Pre-hexon-linking protein VIII"
                     /note="Pre-hexon-linking protein VIII from Human adenovirus
                     C serotype 5. Accession#: P24936"
     CDS             29707..31449
                     /note="Fiber protein from Human adenovirus C serotype 5.
                     Accession#: P11818"
                     /label="Fiber protein"
     CDS             complement(31861..32742)
                     /note="Early 4 ORF6 protein from Human adenovirus C
                     serotype 2. Accession#: P03239"
                     /label="Early 4 ORF6 protein"
     CDS             complement(33021..33368)
                     /note="Early 4 ORF3 protein from Human adenovirus C
                     serotype 2. Accession#: P03241"
                     /label="Early 4 ORF3 protein"
     CDS             complement(33809..34192)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   34502..34604
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(34828..35416)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(35571..36428)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(36429..36533)
                     /label="AmpR promoter"