Basic Vector Information
- Vector Name:
- pAd/PL-DEST
- Antibiotic Resistance:
- Ampicillin
- Length:
- 34864 bp
- Type:
- Gateway Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- lac UV5
pAd/PL-DEST vector Vector Map
pAd/PL-DEST vector Sequence
LOCUS V011865 34864 bp DNA circular UNA 01-JAN-1980
DEFINITION Exported.
ACCESSION V011865
VERSION V011865
KEYWORDS pAd PL-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 34864)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 34864)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..34864
/mol_type="genomic DNA"
/organism="synthetic DNA construct"
repeat_region 1..103
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/label="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
protein_bind 512..636
/label="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 661..691
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 745..1401
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 1746..2048
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2092..2216)
/label="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 2330..2749
/gene="IX"
/label="Hexon-interlacing protein"
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(2815..4149)
/gene="IVa2"
/label="Packaging protein 1"
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
CDS complement(3921..7502)
/gene="POL"
/label="DNA polymerase"
/note="DNA polymerase from Human adenovirus C serotype 2.
Accession#: P03261"
CDS complement(7307..9310)
/gene="PTP"
/label="Preterminal protein"
/note="Preterminal protein from Human adenovirus C serotype
5. Accession#: P04499"
CDS 9771..11015
/gene="L1"
/label="Packaging protein 3"
/note="Packaging protein 3 from Human adenovirus C serotype
5. Accession#: P04496"
CDS 11039..12793
/note="Pre-hexon-linking protein IIIa from Human adenovirus
C serotype 5. Accession#: P12537"
/label="Pre-hexon-linking protein IIIa"
CDS 12877..14589
/gene="L2"
/label="Penton protein"
/note="Penton protein from Human adenovirus C serotype 5.
Accession#: P12538"
CDS 14599..15192
/gene="L2"
/label="Pre-histone-like nucleoprotein"
/note="Pre-histone-like nucleoprotein from Human adenovirus
C serotype 2. Accession#: P68950"
CDS 15265..16368
/gene="L2"
/label="Core-capsid bridging protein"
/note="Core-capsid bridging protein from Human adenovirus C
serotype 5. Accession#: P24938"
CDS 16399..16638
/note="Pre-core protein X from Human adenovirus C serotype
5. Accession#: Q2KS10"
/label="Pre-core protein X"
CDS 16724..17473
/gene="L3"
/label="Pre-protein VI"
/note="Pre-protein VI from Human adenovirus C serotype 5.
Accession#: P24937"
CDS 17563..20418
/gene="L3"
/label="Hexon protein"
/note="Hexon protein from Human adenovirus C serotype 5.
Accession#: P04133"
CDS 20454..21065
/gene="L3"
/label="Protease"
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(21167..22753)
/gene="DBP"
/label="DNA-binding protein"
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 22782..25202
/gene="L4"
/label="Shutoff protein"
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 25895..26575
/gene="L4"
/label="Pre-hexon-linking protein VIII"
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS 27885..29627
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
/label="Fiber protein"
CDS complement(30039..30920)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
/label="Early 4 ORF6 protein"
CDS complement(31199..31546)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
/label="Early 4 ORF3 protein"
CDS complement(31987..32370)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
/label="Early 4 ORF1 protein"
repeat_region 32680..32782
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(33006..33594)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(33749..34606)
/label="AmpR"
/note="beta-lactamase"
promoter complement(34607..34711)
/label="AmpR promoter"
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