pDEST10 vector (V011839)

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LOCUS       pDEST10.        6708 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for expressing N-terminally 
            6xHis-tagged proteins in insect cells using the Bac-to-Bac(R) 
            baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..207
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             262..264
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             274..291
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             313..333
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     protein_bind    337..461
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        608..710
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             711..1367
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1712..2014
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2058..2182)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2410..2544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2573..2738)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2922..3377
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3404..3508
                     /label=AmpR promoter
     CDS             3509..4366
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4540..5128
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5431..5655)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5725..6255)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(6444..6472)
                     /label=Pc promoter
                     /note="class 1 integron promoter"