pDEST26 vector (V011833)

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LOCUS       pDEST26.        7481 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for high-level expression of 
            N-terminally 6xHis-tagged proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST26
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             632..634
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             644..661
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    671..795
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        820..850
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             904..1560
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1905..2207
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2251..2375)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2408..2426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2437..2453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2858..2992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3118..3573
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3692..4049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4100..4891
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4958..5006
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        5312..5416
                     /label=AmpR promoter
     CDS             5417..6274
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6448..7036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"