pENTR1A vector (V011812)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011812	pENTR1A	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:: pENTR1A

Antibiotic Resistance:: Chloramphenicol

Length:: 3754 bp

Type:: Gateway Cloning Vectors

Replication origin:: ori

Source/Author:: Invitrogen (Life Technologies)

Copy Number:: High copy number

pENTR1A vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTR1A vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pENTR1A.        3754 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) Dual Selection Vector for creating an entry vector by 
            restriction cloning. Other reading frames are available with 
            pENTR(TM)2B and pENTR(TM)3C.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR1A.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create a Kozak sequence, clone a blunt fragment starting with 
            ATGG (where ATG is the start codon) into the XmnI site.
FEATURES             Location/Qualifiers
     source          1..3754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    457..507
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site, part 1"
     RBS             461..466
                     /note="ribosome binding site"
     promoter        524..554
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             608..1285
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1608..1910
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_feature    1948..1979
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site, part 2"
     protein_bind    complement(1983..2082)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2205..3011
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3104..3692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"