Basic Vector Information
- Vector Name:
- pENTR2B
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 3755 bp
- Type:
- Gateway Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
pENTR2B vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pENTR2B vector Sequence
LOCUS pENTR2B. 3755 bp DNA circular SYN 01-JAN-1980 DEFINITION Gateway(R) Dual Selection Vector for creating an entry vector by restriction cloning. Other reading frames are available with pENTR(TM)1A and pENTR(TM)3C. ACCESSION . VERSION . KEYWORDS pENTR2B. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3755) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 3755) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT To create a Kozak sequence, clone a blunt fragment starting with ATGG (where ATG is the start codon) into the XmnI site. FEATURES Location/Qualifiers source 1..3755 /mol_type="other DNA" /organism="synthetic DNA construct" terminator 103..189 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" protein_bind 358..457 /label=attL1 /note="recombination site for the Gateway(R) LR reaction" misc_feature 458..508 /label=MCS 1 /note="MCS 1" /note="multiple cloning site, part 1" promoter 525..555 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 609..1286 /label=CmR /note="chloramphenicol acetyltransferase" CDS 1609..1911 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" misc_feature 1949..1980 /label=MCS 2 /note="MCS 2" /note="multiple cloning site, part 2" protein_bind complement(1984..2083) /label=attL2 /note="recombination site for the Gateway(R) LR reaction" CDS 2206..3012 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 3105..3693 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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