pLenti6.3/V5-DEST vector (V011775)

LOCUS       V011775                 9387 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011775
VERSION     V011775
KEYWORDS    pLenti6.3 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2568..2692
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2717..2747
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2801..3457
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3802..4104
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4148..4272)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4325..4366
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    4390..4978
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4993..5309
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5357..5404
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5423..5818
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             5908..6141
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6213..6347
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6374..6509
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6530..6548)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6558..6574)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6716..7171
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7197..7301
                     /label="AmpR promoter"
     CDS             7302..8159
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8333..8921
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9209..9230
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9245..9275
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9283..9299
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9307..9323
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9344..9362
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"