Lafmid BA vector (V011764)

LOCUS       Lafmid_BA.        3152 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Phagemid vector used for cDNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    Lafmid BA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1042..1058
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1070..1101
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(1114..1130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1138..1154)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1162..1192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1207..1228)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1516..2104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2278..3135)
                     /label=AmpR
                     /note="beta-lactamase"