pAMP1 vector (V011762)

LOCUS       pAMP1.        4114 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of a cloned cDNA. For an 
            MCS with FseI and SalI sites, use pAMP10.
ACCESSION   .
VERSION     .
KEYWORDS    pAMP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        164..182
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    191..291
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(305..323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(366..382)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(390..420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(435..456)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(472..1551)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1552..1629)
                     /label=lacI promoter
     rep_origin      complement(1907..2495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2669..3526)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3527..3631)
                     /label=AmpR promoter
     rep_origin      complement(3658..4113)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"