pDNR-Dual vector (V011723)

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LOCUS       40924_14875        4938 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDNR-Dual, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kalnine N, Farmer A, Lin Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella 
            ave., Mountain View, CA 94043, USA
REFERENCE   2  (bases 1 to 4938)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella ave., Mountain
            View, CA 94043, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..42
                     /label=loxP recombination site
                     /note="loxP recombination site"
                     /regulatory_class="other"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    45..129
                     /note="MCS; multiple cloning site"
     misc_feature    132..136
                     /label=splice donor (SD) site
                     /note="splice donor (SD) site"
     CDS             141..176
                     /label=6xHN
                     /note="6x(His-Asn) affinity tag"
     terminator      complement(212..306)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(330..986)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(1015..1048)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1063..1508
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             1509..2927
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        2998..3102
                     /label=AmpR promoter
     CDS             3103..3960
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4134..4722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4839..4855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4863..4881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"