pME18S-FL3 vector (V011718)

LOCUS       pME18S-FL3.        3392 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for directional cloning and transient expression of
            a cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    pME18S-FL3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     A cDNA inserted between the DraIII sites can be excised with XhoI.
FEATURES             Location/Qualifiers
     source          1..3392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..632
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    812..1194
                     /label=stuffer
                     /note="stuffer"
     polyA_signal    1297..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1641..2229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2403..3260)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3261..3365)
                     /label=AmpR promoter