pOTB7-3 vector (V011717)

Download GeneBank File(.gb)

LOCUS       pOTB7-3.        2816 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector with an f1 origin derived from pOTB7.
ACCESSION   .
VERSION     .
KEYWORDS    pOTB7-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The presence in pOTB7-3 of a toxic ccdB gene flanked by BstXI sites 
            is described here:
            http://www.imageconsortium.org/?i=vectors#171
            However, this gene was not detected in the sequence provided.
FEATURES             Location/Qualifiers
     source          1..2816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    444..465
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        480..510
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    518..534
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     542..558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        576..594
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(763..787)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(819..837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(890..1345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1416..2004
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2145..2801
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"