Basic Vector Information
- Vector Name:
- pRKW2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4750 bp
- Type:
- I.M.A.G.E. Consortium Plasmids
- Replication origin:
- ori
- Source/Author:
- I.M.A.G.E. Consortium
- Copy Number:
- High copy number
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pRKW2 vector Map
pRKW2 vector Sequence
LOCUS 40924_37183 4750 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pRKW2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4750) AUTHORS Wu W, Niehrs C. TITLE Direct Submission JOURNAL Submitted (26-MAY-2006) Division of Molecular Embryology (A050), Deutsches Krebsforschungszetrum (DKFZ), Im Neuenheimer Feld 280, Heidelberg 69120, Germany REFERENCE 2 (bases 1 to 4750) TITLE Direct Submission REFERENCE 3 (bases 1 to 4750) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (26-MAY-2006) Division of Molecular Embryology (A050), Deutsches Krebsforschungszetrum (DKFZ), Im Neuenheimer Feld 280, Heidelberg 69120, Germany" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..4750 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 14..393 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 394..597 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 828..846 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(956..972) /label=SK primer /note="common sequencing primer, one of multiple similar variants" polyA_signal 1008..1142 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 1215..1572 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(1592..1608) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1821..2276 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2608..2712 /label=AmpR promoter CDS 2713..3570 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3744..4332 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4620..4641 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4656..4686 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4694..4710 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4718..4734 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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