pSPORT 2 vector (V011711)

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LOCUS       pSPORT_2.        4310 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for cloning, sequencing, and bacterial expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pSPORT 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        164..182
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    191..289
                     /function="multiple cloning site"
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
                     /experiment="experimental evidence, no additional details
                     recorded"
     promoter        complement(303..321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(340..356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(364..380)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(388..418)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(433..454)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(470..1549)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1550..1627)
                     /label=lacI promoter
     rep_origin      complement(1905..2493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(2571..2604)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2709..3566)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3854..4309)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"