pYX-Asc vector (V011708)

Basic Vector Information

Vector Name:
pYX-Asc
Antibiotic Resistance:
Ampicillin
Length:
1691 bp
Type:
I.M.A.G.E. Consortium Plasmids
Replication origin:
ori
Source/Author:
I.M.A.G.E. Consortium
Copy Number:
High copy number

pYX-Asc vector Map

pYX-Asc1691 bp6001200T3 promoterMCST7 promoteroriAmpR

pYX-Asc vector Sequence

LOCUS       pYX-Asc.        1691 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact cDNA cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    pYX-Asc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..1691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..22
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    40..99
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(137..155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(217..805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(817..1674)
                     /label=AmpR
                     /note="beta-lactamase"

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