pAcAB4 vector (V011692)

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LOCUS       V011692                10230 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011692
VERSION     V011692
KEYWORDS    pAcAB4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..578
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2661..3290
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3169..3996
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(4000..4133)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(4462..4571)
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(4722..4813)
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4838..4947
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5000..5091
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     5101..6458
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        8094..8198
                     /label="AmpR promoter"
     CDS             8199..9056
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9230..9818
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    10106..10127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10142..10172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10180..10196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10204..10220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"