pAcGHLT C vector (V011685)

LOCUS       V011685                 8753 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011685
VERSION     V011685
KEYWORDS    pAcGHLT C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2875..2892
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2899..2916
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein
                     kinase A"
                     /label="recognition site for phosphorylation by protein"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2926..2943
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    2958..3025
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     3045..4503
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5647..5663)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5876..6331
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6613..6717
                     /label="AmpR promoter"
     CDS             6718..7575
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7749..8337
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8625..8646
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8661..8691
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8699..8715
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8723..8739
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"