pAcMP2 vector (V011678)

LOCUS       V011678                 9847 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011678
VERSION     V011678
KEYWORDS    pAcMP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4017..4341
                     /label="basic protein promoter"
                     /note="baculovirus basic protein promoter, which allows
                     expression during the late instead of very late phase of
                     viral infection"
     misc_feature    4346..4400
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4862..6317
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7228..7244)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7252..7268)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7276..7306)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7321..7342)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7630..8218)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8392..9249)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9250..9354)
                     /label="AmpR promoter"
     primer_bind     9828..9844
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"