pAcSG2 vector (V011675)

Download GeneBank File(.gb)

LOCUS       pAcSG2.        5544 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact baculovirus transfer vector for expressing proteins using 
            the polyhedrin promoter, for use with the BD BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcSG2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This vector accepts inserts as large as 8 kb.
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..588
                     /label=baculovirus recombination region (ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        592..683
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    690..751
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             710..712
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_recomb     771..2226
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(3137..3153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3161..3177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3185..3215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3230..3251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3539..4127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4301..5158)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5159..5263)
                     /label=AmpR promoter