pBAC-6 vector (V011669)

Download GeneBank File(.gb)

LOCUS       pBAC-6.        5580 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid with the gp64 promoter, encoding an 
            N-terminal signal sequence-6xHis-thrombin-S-Tag-enterokinase 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pBAC-6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        1166..1371
                     /label=gp64 promoter
                     /note="promoter for the baculovirus gp64 glycoprotein gene"
     sig_peptide     1372..1431
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             1441..1458
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1468..1485
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1489..1533
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1549..1563
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    1565..1629
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1630..1647
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     1684..3041
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3304..3892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4066..4923)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4924..5028)
                     /label=AmpR promoter
     rep_origin      5055..5510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"